Figure 7.

Progesterone treatment has an immediate effect on mouse embryo location that resolves over time. Treating the NP with P4 does not affect embryo entry (A). P4 treatment for NP induces an embryo location concordant with a slower unidirectional embryo movement pattern on GD3 1500 h (B) (P < 0.0001 and P < 0.005 for OE and EE distances between vehicle and P4 treatment, respectively). (C) Although the embryos initially display an altered movement pattern, they eventually space out at the end of bidirectional movement on GD3 2200 h. For OE and EE comparison of vehicle and treatment multiple comparisons of means were conducted using Tukey contrasts with the Holm method adjustment for P-values. Each circle represents an embryo, and circles connected with a line are embryos from the same uterine horn. White circles represent mice treated with vehicle and grey circles represent mice treated with P4. The left-hand column indicates the time of dissection in hours (h). ‘N’ represents the number of mice, and ‘Ut’ represents the number of uterine horns analyzed for each time point. Dotted lines divide the uterine horns into three equal segments, and percentages for each time point signify the percentage of embryos in each segment. NP: natural pregnancy; E2: estrogen; P4: progesterone; GD: gestational day.