FIG. 6.

Demonstration of elaboration of mRNA for ExoU in infected lung tissue of mice. RT-PCR of lung tissue from mice infected with either the exoU⁺ PAO1(pUCP19exoUspcU) strain or the parental strain carrying the cloning vector, PAO1(pUCP19). mRNA from tissue was reverse transcribed and amplified with primers specific to the rpoB gene to yield a product of 314 bp or with primers specific to the exoU gene to yield a product of 428 bp. Molecular weight markers on the left are oligonucleotides differing by 100 bp.