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[Preprint]. 2023 Jun 12:2023.04.22.537917. Originally published 2023 Apr 24. [Version 2] doi: 10.1101/2023.04.22.537917
ID Primer Sequence Notes
1 2xStrepTag_rem_F CTCGAAGGCGGCGGGGGA Amplification of 2xStrepTag from pLVX vector
2 2xStrepTag_rem_R TTACTTTTCAAACTGCGGATGTGACCATGATCCAC Amplification of 2xStrepTag from pLVX vector
3 nsp4_Wuhan_xFT_F ATCCGCAGTTTGAAAAGTAATAAACCCGCTGATCAGCC Removal of FLAG tag from nsp4
4 nsp4_Wuhan_xFT_R CATCCCCCGCCGCCTTCGAGCAGGACTGCGGAAGTAATG Removal of FLAG tag from nsp4
5 Wuhan-orf8_F CCGGTGAATTCGCCGCCACCATGAAGTTCCTGGTATTTC Amplification of orf8 gene from pcDNA vector
6 Wuhan-orf8_R CATCCCCCGCCGCCTTCGAGAATAAAGTCCAAGACCAC Amplification of orf8 gene from pcDNA vector
7 pLVX_F CTCGAAGGCGGCGGGGGA Amplication of pLVX vector with 2xStrepTag
8 pLVX_R GGTGGCGGCGAATTCACCG Amplication of pLVX vector with 2xStrepTag
9 GAPDH-qPCR_F GTCGGAGTCAACGGATT
10 GAPDH-qPCR_R AAGCTTCCCGTTCTCAG
11 HSPA5-qPCR_F GCCTGTATTTCTAGACCTGCC
12 HSPA5-qPCR_R TTCATCTTGCCAGCCAGTTG
13 PDIA4-qPCR_F AGTGGGGAGGATGTCAATGC
14 PDIA4-qPCR_R TGGCTGGGATTTGATGACTG