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[Preprint]. 2023 Apr 29:2023.04.28.538728. [Version 1] doi: 10.1101/2023.04.28.538728

Figure 4: Microglia-dendrite interactions shape spine density and morphology.

Figure 4:

(A) Deconvolved confocal image of dendritic branch (green) with the region of microglia-dendrite interaction marked by a magenta box (top). Individual z-slices from the stack (0.1μm steps) with the IBA1+ microglia (in magenta) interacting with the EGFP+ dendrite (green) (bottom). Numbers in upper left hand denote z-steps.

(B) Dendrite proportions that were classified as Touch+ or Touch− across oblique (top) and tuft (bottom) dendrites.

(C) Spine densities (left) and cumulative distributions for spine head diameters (right) from oblique Touch+ and Touch− dendrites. Datapoints represent individual branches (n=5–10/mouse) and analyzed with unpaired t-tests between Touch+ and Touch− branches from B6 (middle) and PWK (right). K-S tests were used to evaluate statistical differences (see Table S6)

(D) Identical analyses to (C) for tuft dendrites. See Table S7 for statistics.

For (C) and (D) *p<0.05, unpaired t-test.