Fig. 5. Locally concentrating NM2 monomer results in filament assembly. (a) Cartoon of iLID optogenetic system with photo-recruitable NM2 monomers expressed with endogenously tagged NM2.

(b) Representative frame of optogenetic recruitment of NM2 monomers in cell. (b,c)Magenta box indicates the zoom inset region, orange circle marks the activated region, and green area shows the direction of retrograde flow. (c) Time series of optogenetic NM2 recruitment with recruitable NM2 in blue/grey and endogenous NM2 in purple/grey. Top panel is the recruitable NM2, middle panel is endogenous, and bottom panel is overlay of the two channels. Scale bar = 10 um.