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[Preprint]. 2023 Sep 6:2023.04.29.538828. Originally published 2023 Apr 29. [Version 2] doi: 10.1101/2023.04.29.538828

Fig 6: Biological significance of altered pathways in ovarian stroma and theca SCLs.

Fig 6:

(A) Expression of collagen genes in the fibroblast-like stroma SCL does not change with age. (B) PSR staining of collagen deposition in 3- and 9-month-old ovaries. (C) IPA upstream regulator analyses of age-related changes in stroma and TC SCLs (9 months-old vs. 3 months-old, z-score) related to inflammation and fibrosis. (D) Expression of Mmp2 gene in the fibroblast-like stroma decreases with age. (E) MMP2 protein is decreased in aged ovarian stroma by immunofluorescence. (F) Representative images of immunofluorescence of MMP2 (green), DAPI (blue) and autofluorescence (red). Yellow lines show representation of stromal regions analyzed, avoiding follicles and auto-fluorescent regions. (G-H) CellChat chord diagrams of the TGF-β signaling pathway interactions in the (G) 3-mo and (H) 9-mo ovarian CLUs. (I) IPA canonical pathways indicating activation of specific pathways by aging in TCs. (J) IPA upstream regulator analyses of age-related changes in stroma and TC SCLs (9 months-old vs. 3 months-old, z-score) related to cell proliferation. (n=5/age). scRNA-seq was performed in n=4 ovaries/age. Data are presented as mean ± SEM. *, **, *** represent statistical difference (p<0.05, 0.01 and 0.005, respectively) by one-tailed t-test (A-B) or two-tailed t test (D-E). Black scale bars represent 500μm and white scale bars represent 100μm.