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[Preprint]. 2023 Apr 24:rs.3.rs-2839029. [Version 1] doi: 10.21203/rs.3.rs-2839029/v1

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The p.E256K mutation alters the kinetics of D-serine uptake. (A) Wildtype or p.E256K mutant human SLC1A4 was expressed in Xenopus oocytes and [3H]-labeled D-serine uptake measured by liquid scintillation spectroscopy. The mutant transporter consistently showed increased affinity for D-serine (Km) but lower maximum velocity (Vmax), indicating saturation at a lower concentration of D-serine. (B) Brain slices from Slc1a4^K/K mice showed higher [3H]-labeled D-serine (100 nM) uptake than Slc1a4^E/E (wildtype) brain slices, consistent with the mutant transporter change in D-serine kinetic parameters.