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. 2023 Apr 25;11:1133122. doi: 10.3389/fbioe.2023.1133122

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Copyright © 2023 Wang, Shakeel, Salih, Vurivi, Daoud, Desidery, Khan, Shibru, Ali, Butt, Chan and Corridon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A schematic of the high-throughput system used to generate corneal tissues from slaughterhouse waste. Photographs in panel (A) illustrate the whole eye allocation and large-batch decellularization processes post-slaughter. Photographs in panel (B) highlight the retention of ocular transparency in native corneas using an index-matching agent (glycerol), while those in panel (C) display the reversal of ocular opacity in decellularized tissues using this clearing agent. Opacity is an established consequence of certain forms of corneal extraction and storage, and decellularization.