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. 2023 May 1;16(5):865–881. doi: 10.1016/j.molp.2023.03.015

Figure 2.

Figure 2

rfo1-1 mutant shows altered plant response to perturbation of pectin methylation at the cell wall.

(A) Representative image of 8-day-old WT (Col-0), rfo1-1, and RFO1-GFP (rfo1-1 pRFO1::RFO1-GFP) seedlings after a 48-h Mock (−) or 6.25 μM EGCG (+) treatment. Scale bar, 5 mm.

(B) Root growth of seedlings exposed to EGCG relative to Mock-treated ones as depicted in (A). Data represent the mean ± SE of N ≥ 38 seedlings per genotype from four independent replicates. One-way ANOVA with Dunnett’s multiple-comparison test, ∗∗∗∗p < 0.0001. Significance shown compared with WT.

(C and D) Representative images of WT, PMEIox, rfo1-1, rfo1-1 PMEIox, and rfo1-1 RFO1-GFP PMEIox 8-day-old seedlings (C) or 8-week-old plants (D). In (C), scale bar, 5 mm.

(E) Determination of the degree of methylesterification of pectins using FTIR in 8-day-old WT and rfo1-1 roots. Values correspond to means ± SE of three biological replicates, each one containing two technical replicates.