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. 2023 May 1;16(5):865–881. doi: 10.1016/j.molp.2023.03.015

Figure 5.

Figure 5

rfo1-1 has altered basal and dmPectin-induced MAPK phosphorylation levels and gene expression activation.

(A) Quantification of relative MAPK activation in blots as shown in Supplemental Figure 8A. Within each blot, relative MAPK activation was determined by calculating active to total MAPK intensities per sample, and these active/total data were normalized to the untreated WT. Data represent the mean ± SE of N = 5 independent replicates. Multiple-comparison t-tests were performed for WT or rfo1-1 samples with respect to their mock-treated control (black) or between WT and rfo1-1 samples (blue), ∗p < 0.05, ∗∗∗p < 0.001.

(B)WAK2, WRKY45, WRKY53, JAZ10, DWF4, PAD4, and RFO1 gene expression relative to GAPDH in 8-day-old roots of Mock-, mPectin-, or dmPectin-treated WT and rfo1-1 plants. The expression values were then normalized to Mock WT. Data represent the mean ± SE of N = 3 independent replicates. Data were analyzed by t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Asterisks in black indicate the difference compared with WT Mock. Asterisks in blue indicate the differences between rfo1-1 Mock versus rfo1-1 treatment (mPectin or dmPectin).