Figure 1.

Co-recruitment and co-displacement of PRC2.1 and PRC2.2 during ESC to EpiLC differentiation

(A) Top: schematic of differentiation model. Bottom: bar plots showing the expression of ESC marker genes Prdm14 and Klf4, and EpiLC marker genes Fgf5 and Dnmt3b by qPCR (n = 2) and RNA-seq (n = 3). Error bars represent SD.

(B) Left: heatmap representing fold change in SUZ12 binding at PRC2 target promoters in ESC versus EpiLC cells. Indicated are three categories of PRC2 targets—displaced SUZ12 (log₂FC < −1 and p value < 0.05; n = 78), maintained SUZ12 (n = 2,175), and recruited SUZ12 in EpiLC cells (log₂FC > 1 and p value < 0.05; n = 398). Right: tornado plots showing enrichments of indicated antibodies at displaced, maintained, and recruited promoters in ESCs and EpiLCs.

(C) Genome browser tracks showing ChIP-Rx for the indicated antibodies and RNA-seq profiles in ESC and EpiLC cells at Epcam (displaced), Sox8 (maintained), and Tbx3 (recruited).

(D) Boxplots presenting mRNA abundance of displaced, maintained, and recruited PRC2 target genes. ^∗∗∗p value < 0.001.

See also Figure S1 and Table S1.