Figure 2.

PRC2.1 drives H3K27me3 deposition while PRC2.2 drives CBX7-cPRC1 recruitment to Polycomb target genes

(A) Schematic of ESC lines used.

(B) Tornado and average plots showing ChIP-Rx enrichments for the indicated antibodies at recruited Polycomb target genes (n = 398 sites) in WT and mutant EpiLCs.

(C) Genome browser tracks showing ChIP-Rx profiles of the indicated antibodies in WT and mutant EpiLCs at the maintained Six3 and Six2 gene loci.

(D) Line plots representing ChIP-Rx enrichment of indicated antibodies in WT and mutant EpiLCs, relative to their respective levels in WT EpiLCs.

(E) Western blot of the indicated antibodies on cytoplasm, nucleosol, or chromatin fractions of the indicated cell lines.

See also Figure S2.