Figure 5.
Contrasting PRC2.1 and PRC2.2 binding profiles consistent with independent recruitment mechanisms
(A) Genome browser tracks showing SUZ12 and CBX7 ChIP-Rx binding in the relevant cells at the HoxC locus. The red region represents recruited genes, whereas the gray region represents a group of maintained genes.
(B) Genome browser tracks of the indicated antibodies in the indicated cell lines. Bio-CAP tracks generated on wild-type ESCs, taken from GSE43512.65
(C) Left: schematic of assay design. Right: boxplot representing the distance between the SUZ12 peak center of WT, J2KO, and TKO EpiLCs and the center of the CGIs. ∗∗∗p value < 0.001.
(D) Genome browser tracks of the indicated antibodies in the indicated cell lines at the extended HoxC locus.
(E) Genome browser tracks of the indicated antibodies in the indicated cell lines, as well as Bio-CAP, the extended HoxC locus.
(F) Boxplot representing the distance between the center of SUZ12 peaks in WT, and in QKO + FLAG-MTF2 or QKO + FLAG-JARID2.
See also Figure S5.