Figure 6.

DNA and histone modification binding activities of MTF2 and JARID2 facilitate the respective chromatin binding of PRC2.1 and PRC2.2

(A) Schematic of wild-type or mutant MTF2 rescue strategy.

(B) Western blot analyses using the indicated antibodies of total protein extracts in the indicated ESC lines.

(C) Boxplot of SUZ12 ChIP-Rx read counts in the indicated ESC lines at all PRC2-bound promoters. ^∗∗p value < 0.01.

(D) Genome browser tracks showing SUZ12 ChIP-Rx profile on the HoxA locus in the indicated cell lines. Note the y axis values are adjusted to facilitate the visualization of the tracks.

(E) Schematic of wild-type or truncated JARID2 rescue strategy.

(F) Western blot analyses using the indicated antibodies on total protein extracts in the indicated ESC lines.

(G) Genome browser tracks showing SUZ12 ChIP-Rx profile in the indicated cell lines at representative UIM-dependent (top) and UIM-independent (bottom) gene loci.

(H) Left: heatmap representing the fold change of SUZ12 binding at PRC2 target promoters in QKO + JARID2-WT or QKO + JARID2-ΔUIM. Right: average plots showing ChIP-Rx normalized read densities for SUZ12 and CBX7 at the UIM-dependent (n = 300) and UIM-independent (n = 300) regions, in QKO and JARID2 rescue ESCs.

See also Figure S6.