Figure 7.

PRC2.1-deposited H3K27me3 and PRC2.2-JARID2-recruited CBX7-cPRC1 cooperate to mediate Polycomb target repression

(A) Genome browser tracks showing 4C-seq analyses of the indicated cell lines using the Tbx3 gene promoter as the viewpoint bait. SUZ12 and H3K27ac ChIP-Rx profiles are shown below. SUZ12-bound sites (S1, S2, and S3) are highlighted in blue, while the H3K27ac-enriched enhancers (E1, E2, and E3) are highlighted in pink.

(B) Boxplots representing the 4C-seq densities at S1, S2, and S3 and E1, E2, and E3 in the indicated cell lines. ^∗∗p value < 0.01.

(C) Genome browser tracks showing 4C-seq analyses of the indicated cell lines using the Tbx3 gene promoter as the viewpoint bait. ChIP-Rx profiles of the indicated antibodies in the relevant cell lines are also shown.

(D) Relative mRNA abundance of Tbx3 in WT ESCs, and WT and Pcgf2/4 KO EpiLCs. Error bars represent SD (n = 3).

(E) Bar plots representing the fold change of Tbx3 expression between WT and PcG mutant EpiLCs. ^∗∗∗p value < 0.001. Error bars represent SD (n = 3).

(F) Bar plots representing the number of DESeq2-identified differentially expressed recruited Polycomb target genes in the indicated EpiLCs (n = 3).

(G) Boxplots showing RNA-seq log₂-fold change of WT compared with the respective KO EpiLCs. The gray dashed line indicates the y axis at 0. ^∗p value < 0.05 and ^∗∗p value < 0.01. Error bars represent SD (n = 3).

See also Figure S7.