Table 1.
Amino acid substitutions accumulated during three passages of SIVmac239 in hu-mice.
| Protein | Position | Stock Residuea |
Variant Residueb |
Stock Frequencyc |
P3 Endpoint Frequencyd |
P3 Endpoint Frequencye |
|---|---|---|---|---|---|---|
| Gag | 216 | L | V | 0.00f | 1.00 | 1.00 |
| Pol | 821 | A | T | 0.00f | 1.00 | 0.94 |
| Env | 67 | V | M | 0.00f | 1.00 | 1.00 |
| Env | 481 | T | A | 0.00f | 0.85 | 0.84 |
| Env | 676 | D | E | 0.00f | 0.95 | 0.23 |
| Nef | 193 | Y | C | 0.00f | 1.00 | 1.00 |
| Nef | 221 | Y | N | 0.00f | 1.00 | 1.00 |
| Nef | 258 | D | N | 0.00f | 1.00 | 0.15 |
a
Consensus amino acid residue from sequenced stock virus.
b
Variant amino acid residue from passaged virus.
c
Frequency of variant residue in the stock virus population.
d
Replicate 1 frequency of variant residue at the end of the third passage.
e
Replicate 2 frequency of variant residue at the end of the third passage.
f
The 0.00 indicates that the variant frequency was below the limit of detection of the variant identification pipeline.