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. Author manuscript; available in PMC: 2023 May 9.
Published in final edited form as: ACS Catal. 2022 Nov 14;12(23):14559–14570. doi: 10.1021/acscatal.2c04232

Figure 3. Eosin Y/sodium sulfite photo-reduction system for Stc2 reaction.

Figure 3.

a), Scheme of Stc2-catalyzed N- demethylation reaction with eosin Y as the photo-sensitizer and sulfite as the sacrificial reagent. b), Time course of Stc2 reduction under various eosin concentrations. The reaction mixture contained 2 mM stachydrine, 100 μM Stc2, 0.15 – 0.6 μM of eosin Y, and 20 mM sodium sulfite in 20 mM Tris, pH 8.0 buffer. c), Stc2 reduction time-course monitored by UV-visible spectroscopy. The UV spectra were collected at 0 min (red trace) and 40 min (dark blue trace). The same mixture without Stc2 is set up as control (black trace, with 0.3 μM of eosin Y) to show the change of UV-visible spectra during the photoreduction process.