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. Author manuscript; available in PMC: 2023 May 9.
Published in final edited form as: ACS Catal. 2022 Nov 14;12(23):14559–14570. doi: 10.1021/acscatal.2c04232

Figure 4. Further optimization of Stc2 photo-biocatalysis and a comparative characterization between the Stc2-photo-biocatalysis and the Stc2-NAD(P)H-reductase system.

Figure 4.

a), Flow-chemistry setting in Stc2-catalysis by separating the photo-reduction half-reaction and the oxidation half-reaction. To address the O2 delivery challenge in flow-chemistry, chlorite dismutase (CLD) mediated in situ O2 production was further introduced into Stc2-catalysis. b), a representative 1H-NMR spectrum [the methyl group signals for substrate (1) and product (2) in 1H-NMR] of Stc2 reaction under the multiple turnover reaction condition using eosin Y/Na2SO3 photo-reduction system (trace i) in batch reaction; under an optimized eosin Y concentration flow-chemistry setting, which further improved the Stc2 turnover number to ~65-70 (trace ii); and Stc2 reaction using GbcB as the reductase and NADH as the reductant (trace iii).