FIG. 3.

Effect of immunization with BeSO₄ and IL-12 on footpad swelling after L. major infection in BALB/c mice. Eight groups of mice (four or five mice per group) were immunized: PBS (control), SLA (group i), SLA and 0.1 μg of IL-12 (group v), SLA and 0.25 μg of IL-12 (group iii), SLA and 1.0 μg of IL-12 (group ix), SLA plus BeSO₄ and 0.1 μg of IL-12 (group vii), SLA plus BeSO₄, and 0.25 μg of IL-12 (group iv), and SLA plus BeSO₄, and 1.0 μg of IL-12 (group x). As described in Materials and Methods, the mice were boosted with the same regimen 7 days after initial immunizations and challenged with parasites 2 weeks thereafter. For all lesion sizes greater than 1.0 mm, the average coefficient of variance was 0.27 (range, 0.14 to 0.37). For all lesion sizes less than 1.0 mm, the average standard deviation was 0.14 (range, 0.00 to 0.58). Each data point represents the mean of lesion sizes for four or five mice. The F test was used for comparisons.