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. 2023 May 8;18:339. doi: 10.1186/s13018-023-03818-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Identification of ADSCs. A The morphology of ADSCs observed under a light microscope; B the results of alizarin red staining after ADSCs cultured for osteogenic induction. C The results of Oil red O staining after ADSCs cultured for adipogenic induction. D The results of Alcian blue staining after ADSCs cultured for chondrogenic induction. E–H: The positive rate of ADSCs antigens CD29 (E), CD31 (F), CD90 (G), and CD45 (H) detected by flow cytometry