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Immunoblot analysis of the RgpA-Kgp complex using murine anti-RgpA-Kgp complex sera. The RgpA-Kgp complex was separated by SDS-PAGE and transferred onto PVDF membrane. The RgpA-Kgp complex was probed with sera (1:50 TN buffer) from mice immunized with the RgpA-Kgp proteinase complex. Molecular mass markers are indicated in kilodaltons.
