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. 2023 Apr 26;14:1168589. doi: 10.3389/fimmu.2023.1168589

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Copyright © 2023 Lagisquet, Conrad, Wittmann, Volkmann, Weissinger, Sticht and Gramberg

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRIM5α H43Y is less active against exogenous retroviruses in vitro. (A) CRFK cells stably expressing empty vector or HA-tagged human or rhesus (rh) TRIM5α variants were generated. Expression of the different HA-TRIM5a constructs was analyzed by immunoblot. Empty vector cells or CRFK cells stably expressing the indicated TRIM5α proteins were challenged with (B) VSV-G pseudotyped HIV-1-GFP reporter virus or (C) N-tropic Murine Leukemia Virus encoding GFP (N-MLV-GFP) at the indicated multiplicities of infection (MOI). Three days postinfection (dpi), the percentage of GFP-positive cells were quantified by flow cytometry and is shown as mean of triplicate infections with error bars indicating SD. One out of three independent experiments is shown. Statistical analysis were done using two-way ANOVA followed by Bonferroni’s multiple comparison test. **P<0.01, ****P<0.0001.