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. 2023 Apr 26;14:1168589. doi: 10.3389/fimmu.2023.1168589

Figure 5.

Figure 5

LINE-1 promoter activity is strongly repressed by TRIM5α H43Y. 293T-shTRIM5α cells were transfected with a LINE-1 promoter-driven luciferase reporter plasmid (L1 5’UTR-luc) together with (A) increasing amounts of TRIM5α WT or H43Y, or (B) 25 ng of TRIM5α WT or H43Y and increasing amounts of CMV-LINE-1. Two days posttransfection, cells were lysed and LINE-1 promoter activity was determined by luciferase assay. Relative luminescence units (RLUs) are shown as mean of quadruplicate transfections with error bars indicating SD. Statistical analysis were done using two-way ANOVA followed by (A) Bonferroni’s multiple comparison test or (B) Tukey’s multiple comparison test, *P<0.1, **P<0.01, ***P<0,001 ****P<0.0001, ns, not significant. One out of three independent experiments is shown. R shRNA-resistant TRIM5α WT or H43Y.