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. 2023 Apr 26;14:1162828. doi: 10.3389/fpls.2023.1162828

Figure 3.

Figure 3

Cloning of BOS1 gene through positional cloning and MutMap strategies. (A) Positional cloning of BOS1 gene. Upper and middle panels represent crude and fine mapping results of bos1-1 mutation, respectively. The numbers of plants with bos1-1 mutant phenotypes used and the molecular markers are shown. Lower panel shows the gene structure of BOS1. Black line represents exons. Gray lines represent the 5′ untranslated region (5′UTR) and 3′UTR. (B) SNP index obtained by MutMap through high-throughput sequencing. (C) Sequence alignment and the trace file showing the mutation identified by sequencing in WT and bos1-1. (D) Photos of panicles in WT, bos1-1, and three complementary transgenic lines. Scale bar is 1 cm. (E, F) The number of PBs (E), SBs (F), and grains (G). (H) Sequence alignment of BOS1 protein and other 17 closely homologous bHLH proteins. SNP, single-nucleotide polymorphism; WT, wild type; PBs, primary branch; SBs, secondary branches. Data in E-G are means ± s.d.. The s.d. represents standard deviation. A two-tailed unpaired t-test with Welch’s correction is used for statistical analysis. Asterisks indicate significant differences (**P < 0.01; Student’s t-test).