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. 2023 Apr 26;14:1162828. doi: 10.3389/fpls.2023.1162828

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Copyright © 2023 Lv, Zhang, Hu, Liu, Yin and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular localization of BOS1 protein and analysis of BOS1 gene expression profiles. (A) Representative microscopic images showing the subcellular localization of green fluorescent protein (GFP) and BOS1-GFP fusion protein transiently expressed in Nicotiana benthamiana leaves driven by 35S promoter. DAPI was used as a nuclear marker. Scale bars are 20 and 10 μm as labeled. (B) Spatial–temporal of BOS1 gene expression of various tissues/organs throughout entire growth in the field. Three biological replications were performed. (C) Hormone response elements in the promoter region of BOS1. Triangles in different colors represent the elements identified. (D) Heat map of BOS1 gene expression pattern under auxin, GA, CK, BR, ABA, and JA treatment. Two biological replications were performed. CK, cytokinin; BR, brassinosteroid; ABA, abscisic acid; JA, jasmonic acid.