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. 2023 May 9;14:2463. doi: 10.1038/s41467-023-37863-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Diagram of the SNAP-synNotch receptor. b Lentiviral vector design for SNAP-synNotch receptor expression and the corresponding reporter gene. The SNAP- synNotch receptor contains the Gal4-VP64 transcription factor which upon activation leads to activation of the TagBFP reporter. c Flow cytometry analysis of surface expression and enzymatic functionality of the SNAP-synNotch receptor on transduced vs. mock (non-transduced) Jurkat cells assessed by staining with an anti-mycTag antibody and a SNAP-Surface-AF647 dye (arbitrary units, arb. units). d Flow cytometry analysis of the activation of SNAP-synNotch Jurkat cells co-incubated with the indicated target cell lines and antibody concentrations for TagBFP output gene expression reported as mean fluorescence intensity (MFI) gated on mCherry+ cells and e by ELISA for the production of the IL-7 therapeutic transgene. For d and e, n = 3 biologically-independent experiments ± s.e.m. Source data are available as a Source Data file.