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. 2023 May 9;14:2463. doi: 10.1038/s41467-023-37863-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a SNAP-CAR design. b SNAP-CAR lentiviral expression construct. c Flow cytometry analysis of the expression and enzymatic functionality of the SNAP-CAR receptor on transduced vs. mock (nontransduced) Jurkat cells, assessed by staining with SNAP-Surface-AF647 dye and recording TagBFP expression (arbitrary units, arb. units). d Flow cytometry analysis of CD25 and CD62L T cell activation markers on Jurkat SNAP-CAR effector cells (gated by TagBFP+ expression) co-incubated with the indicated target cell lines and antibody concentrations reported as mean fluorescence intensity (MFI). CD25 increases while CD62L decreases with activation, n = 3 biologically independent experiments; averages ± s.e.m. Source data are available as a Source Data file.