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. 2023 May 9;14:2463. doi: 10.1038/s41467-023-37863-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Flow cytometry analysis of SNAP receptor activation for SNAP-synNotch and SNAP-CAR cells co-incubated with target cells that were pre-labeled with the indicated antibodies. b Flow cytometry analysis of SNAP receptor activation for SNAP-synNotch and SNAP-CAR cells that were pre-labeled with the indicated antibodies and co-incubated with target cells. TagBFP output gene expression and CD25 marker expression were evaluated by flow cytometry. c Specific lysis of target cells by co-incubated primary human SNAP-CAR T cells that were pre-incubated with the indicated concentration of adaptor at the indicated cell concentration as compared to SNAP-CAR T cells incubated with 1.0 μg/mL of soluble adaptor or a positive control anti-CD20 CAR. For a and b, two-way ANOVA tests with multiple comparisons were performed. As the data did not have homogeneity of variance (Levene’s test), Tukey’s HSD was used for post-hoc analysis between antibody conditions. “ * ” denotes a significance of p < 0.0001, n = 3 biologically-independent experiments ± s.e.m. For c a one-way ANOVA with Dunnet’s Multiple Comparison tests was performed and all values were significantly different (p < 0.0001) from the no adaptor control (white bar). Source data are available as a Source Data file.