Table 3.
Strains of Malassezia pachydermatis used, including animal host, health status, corresponding type of ERG11 sequence, amino acid substitutions, mean inhibition diameters of the disk diffusion method and itraconazole minimum inhibitory concentration (MIC) using E-test method at 72 h
| Strain | Host | Health status | ERG11 DNA Sequence type | Amino acid substitutions | Mean inhibition diameters (mm) values at 72 h | MIC (µg/ml) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| FLZ (R) | FLZ (B) | ITZ (R) | KTZ (R) | KTZ (B) | E-test ITZ | |||||
| CBS1879 | Dog | OE | Ia | – | 28 | 36.5 | 37.5 | 49 | 54.5 | 0.002 |
| CBS6535 | Dog | H | Ia | – | 26 | 36.5 | 34 | 50 | 55 | 0.003 |
| MA13 | Dog | H | Ia | – | 36 | 36 | 40 | 53 | 63 | 0.003 |
| MA52 | Dog | H | Ia | – | 34 | 37 | 38.5 | 51.5 | 58.5 | 0.002 |
| MA56 | Dog | H | Ia | – | 35 | 35.5 | 38 | 47.5 | 59.5 | 0.003 |
| MA195 | Dog | OE | Ia | – | 56.5 | 47 | 57 | 64 | 63.5 | 0.002 |
| MA1595 | Cow | H | Ia | – | 36 | 39 | 35 | 49.5 | 57 | – |
| CBS1884 | Dog | OE | IIa | E181Q | 35 | 42.5 | 42 | 51.5 | 69.5 | 0.002 |
| MA94 | Horse | H | IIa | E181Q | 31.5 | 39 | 34.5 | 49 | 53 | 0.002 |
| MA312 | Cat | OE | XIa | E181Q | 36 | 38.5 | 37.5 | 48.5 | 62 | 0.003 |
| MA587 | Cat | OM | IIa | E181Q | 37.5 | 54.5 | 36.5 | 46.5 | 38.5 | – |
| MA579 | Cat | D | XVIIIa | I25V, E181Q | 31.5 | 41 | 40 | 54 | 67 | 0.002 |
| MA1716 | Dog | D | XXIa | A302T, G459D | – | 6.5 | – | – | 59 | 0.125 |
| MA10 | Dog | OE (C) | VIIIa | I25V, E181Q, T354I | 31 | 28 | 58 | 47 | 49 | – |
| MA140 | Cat | H | IVa | I25V, E181Q, T354I | 32.5 | 28 | 42 | 47 | 56 | 0.002 |
| MA1401 | Dog | OE (R) | XVIa | V33I, E181Q, R202H | 23 | 30 | 34 | 45 | 60 | – |
| MA475 | Pig | H | Vb | R84K, D166E, E181Q, D405N | 29 | 30.5 | 29.5 | 44 | 53 | 0.002 |
| MA7 | Dog | OE (R) | VIb | A17T, R84K, R175H, Q178R, E181Q | 21 | 17.5 | 30 | 50 | 61 | – |
| MA8 | Dog | OE (C) | VIIb | A17T, R84K, R175H, Q178R, E181Q | 40.5 | 41.5 | 45.5 | 59 | 60 | – |
| MA856 | Dog | OE (A) | XIVb | A17T, R84K, R175H, Q178R, E181Q | 20 | 24 | 33 | 39.5 | 63.5 | – |
| MA944 | Dog | OE (C) | XIVb | A17T, R84K, R175H, Q178R, E181Q | 15.5 | 27.5 | 29 | 45 | 59 | – |
| MA968 | Dog | OE (P) | XIVb | A17T, R84K, R175H, Q178R, E181Q | 25 | 25 | 25 | 44.5 | 68.5 | – |
| MA1382 | Dog | OE (C) | XVb | A17T, R84K, R175H, Q178R, E181Q | 16 | 18.5 | 41 | 54 | 59.5 | 0.002 |
| MA107 | Goat | H | IIIc | E181Q, N212S, E290D, Y352F, H399R | – | – | – | – | – | – |
| MA165 | Dog | OE (C) | IXb | A17T, R84K, F143S, R175H, Q178R, E181Q | 21 | 33.5 | 19 | 20 | 66.5 | 0.002 |
| MA356 | Dog | O | XIIb | A17T, R84K, Q178R, E181Q, Y352F, H399R | 23.5 | 27.5 | 39 | 51 | 53.5 | 0.002 |
| MA280 | Dog | OE (C) | Xc | I25S, W52L, R84K, L86F, E181Q, N212S, E290D, Y352F, H399R | 24.5 | 9.5 | 46 | 62.5 | 64 | 0.002 |
| MA361 | Dog | OE (C) | XIIIc | I25S, W52L, R84K, L86F, E181Q, N212S, E290D, Y352F, H399R | 18 | 20 | 34 | 51 | 63 | – |
| MA1289 | Dog | D | XIXc | I25S, W52L, R84K, L86F, E181Q, N212S, E290D, Y352F, H399R | 19 | 19 | 40 | 52.5 | 65 | – |
| MA1478 | Dog | OE (A) | XVIIc | I25S, W52L, R84K, L86F, E181Q, N212S, S226L, E290D, Y352F, H399R | 8 | 5.5 | 32 | 59 | 58.5 | – |
| MA1429 | Dog | D | XXc | I25S, W52L, R84K, L86F, E181Q, N212S, E290D, A306S, Y352F, H399R, G461D | 0 | 0 | 32.5 | 47 | 64.5 | – |
In bold, deleterious mutations
OE, otitis externa; OM, otitis media; A, acute; C, chronic; P, purulent; R, recurrent; H, healthy; D, dermatitis; FLZ, fluconazole; ITZ, itraconazole; KTZ, ketoconazole; R, Rosco©; B, Bio-Rad©
Subclades from the phylogenetic analysis of ERG11 gene: asubclade I; bsubclade III; csubclade II
– Not tested