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. 2023 Jan 1;39(5):745–758. doi: 10.1007/s12264-022-01011-8

Fig. 1.

Fig. 1

Ntn-3 is expressed in DRG sensory neurons and its expression is inversely correlated with the severity of DNP. A Immunostaining of Ntn-3 colocalized with NeuN (arrows) in the DRGs of adult WT mice. Scale bar, 50 μm. The boxed region of the merged images is enlarged on the right. Scale bar, 15 μm. B Co-immunostaining of Ntn-3 and neuronal subtype markers in mouse DRGs. Ntn-3 is expressed in NF200+ and CGRP+ neurons (arrows), but rarely in IB4+ neurons. Scale bar, 50 μm. C The ratio of neuronal subtypes in total Ntn-3+ neurons. n = 3 mice. D Schematic of the experimental design. E Weekly blood glucose tests show that hyperglycemia is induced by STZ administration in mice. **P < 0.01; ns, no significant difference by Student’s t-test. n = 9 mice per group. F qPCR analysis show that Ntn-3 expression is downregulated in the DRGs of DNP mice. **P < 0.01. ns, no significant difference by Student’s t-test. n = 9 mice per group. G Ntn-3 expression in DRGs is significantly correlated with the mechanical pain threshold in diabetic mice at 4 weeks after STZ administration. n = 12 mice.