Table 1.
Primers and probe target sequences.
| Set | Forward sequence | Reverse sequence | Probe sequence | Probe fluorophore |
|---|---|---|---|---|
| 69/70 | CTGCATACACTAATTCTTTCAC | GGTCCCAGAGACATGTATAG | CAACTCAGGACTTGTTCTTACCTT | FAM |
| 241–3 | AATTTAGTGCGTGATCTCCC | AACTTCTATGTAAAGCAAGTAAAG | TTGCCAATAGGTATTAACATCACTAGGT | FAM |
| K/T417 | CTTTTAAGTGTTATGGAGTGTT | CTGGTAATTTATAATTATAATCAGCAATC | CTCTGCTTTACTAATGTCTATGCAGATTCAT | HEX |
| 501Y | GGTTTCCAACCCACTT | AGACTTTTTAGGTCCACAAAC | TTACCAACCATACAGAGTAGTAGTACTTTC | HEX |
| P681RT715wt | TCAGACTCAGACTAATTCTCG | CATAGACACTGGTAGAATTTCTG | CTCTATTGCCATACCCACAAATTTTAC | FAM |
| A222 | AGGGTTTTTCGGCTTTAGAA | AACTTCTATGTAAAGCAAGTAAAG | AGGGTTTTTCGGCTTTAGAA | FAM |
| L452R | GTTGGTGGTAATTATAATTACCG | CATATGATTGTAAAGGAAAGTAACA | CCGGTAGCACACCTTGTAATG | HEX |
| RPP30 | AGATTTGGACCTGCGAGCG | GAGCGGCTGTCTCCACAAGT | TTCTGACCTGAAGGCTCTGCGCG | HEX |