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Specific signal pattern for each variant in the FAM and HEX channels. For each variant, targets that are expected to be amplified are noted by “YES”, and targets that are expected to not be amplified are marked by “no”. Fluorescent signals can be low or high based on probe concentrations. Variants with * indicate expected signals determined by in-silico analysis and were not confirmed with actual samples. Cells in grey indicate hallmark mutations required for identification.
