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. 2023 May 10;13:7582. doi: 10.1038/s41598-023-34643-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of aptamers on the survival of P. aeruginosa. (A) Representative images of drop plate method for analysis of antibacterial activity of aptamers on P. aeruginosa growth. Each plate represents different time points (0, 1 and 2 h) starting from the dilution 10⁴ (right) to 10⁹ (left). (B) Colony forming unit (CFU/mL) of P. aeruginosa in the presence and absence of 10 µM of aptamers (n = 5). (C) Percentage of P. aeruginosa growth after 1- and 2-h incubation with and without aptamers. (D) Concentration of DNA released by P. aeruginosa after 2 h incubation with 10 μM of aptamers indicating leakage of cytoplasmic materials due to membrane damage (n = 3). Data represent an average of three independent experiments, ± SD shown by error bar; *p value of < 0.05, and **p-value of < 0.01.