Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-G3BP1 | Cell Signaling | Cat #17798 |
| Mouse anti-G3BP1 | Santa Cruz Biotechnology | Cat #sc-365338 |
| Rabbit anti-RIG-I | In-house | Takashi Fujita lab (Oh et al., 2016) |
| Rabbit anti-MAVS | Fortis Life Sciences | Cat #A300-782A |
| Mouse anti-TIAR | Santa Cruz Biotechnology | Cat #sc-398372 |
| Rabbit anti-phospho-IRF3 | Cell Signaling | Cat #4947 |
| Rabbit anti-IRF3 (WB) | Cell Signaling | Cat #4302 |
| Rabbit anti-IRF3 (IF) | In-house | Takashi Fujita lab (Oh et al., 2016) |
| Rabbit anti-beta-actin | Cell Signaling | Cat #4302 |
| Mouse anti-vinculin | Cell Signaling | Cat#4650S |
| Rabbit anti-UBAP2L | Fortis Life Sciences | Cat #A300-533A |
| Rabbit anti-PKR | Cell Signaling | Cat #12297 |
| Rabbit anti-G3BP2 | Fortis Life Sciences | Cat #A302-040A |
| Rabbit anti-NIX | Cell Signaling | Cat #12396 |
| Rabbit anti-COXIV | Cell Signaling | Cat #4850 |
| Rabbit anti-VCP | Abcam | Cat #ab111740 |
| Rabbit anti-NDP52 | Abcam | Cat #ab68588 |
| Rabbit anti-MDA5 | Cell Signaling | Cat #5321 |
| Mouse anti-TRAF2 | Santa Cruz Biotechnology | Cat #sc-136999 |
| Mouse anti-TRAF6 | Santa Cruz Biotechnology | Cat #sc-8409 |
| Rabbit anti-TBK1 | Cell Signaling | Cat #3054 |
| Mouse anti-puromycin | EMD Milipore | Cat #MABE343 |
| Rabbit anti-PKR | Cell Signaling | Cat #12297 |
| Mouse anti-OAS3 | Santa Cruz Biotechnology | Cat #sc-398225 |
| Rabbit anti-RNase L (WB) | Cell Signaling | Cat #2728 |
| Mouse anti-RNase L (IF) | Santa Cruz Biotechnology | Cat #sc-74405 |
| Rabbit anti-phospho-PKR | Abcam | Cat #ab32036 |
| Rabbit anti-ATF4 | Cell Signaling | Cat #11815 |
| Rabbit anti-Caspase-8 | Cell Signaling | Cat #4790 |
| Rabbit anti-Caspase-8 | Sigma Aldrich | Cat #NB100-56116 |
| Rabbit anti-Caspase-9 | Cell Signaling | Cat #3493 |
| Rabbit anti-Caspase-3 | Cell Signaling | Cat #9662 |
| Rabbit anti-PARP | Cell Signaling | Cat #9532 |
| Rabbit anti-Sendai Virus pAb | MBL | Cat #PD029 |
| Rabbit anti-Influenza Virus A NP pAb | Thermo Fisher | Cat #PA5-32242 |
| Mouse anti-VSV-G | Kerafast | Cat #EB0010 |
| Mouse anti-ADAR1 | Santa Cruz Biotechnology | Cat # sc-73408 |
| Rabbit anti-Caspase-1 | Cell Signaling | Cat #2225 |
| Anti-Rabbit IgG-HRP | Cell Signaling | Cat #7074P2 |
| Anti-Mouse IgG-HRP | GE Healthcare | Cat #NA931V |
| Alexa Fluor 647® Affinipure Donkey anti-Rabbit IgG | Jackson ImmunoResearch Laboratories | Cat #711-605-152 |
| Alexa Fluor® 488 Affinipure Donkey anti-mouse IgG | Jackson ImmunoResearch Laboratories | Cat #715-545-150 |
| Alexa Fluor® 488 Affinipure Donkey anti-rabbit IgG | Jackson ImmunoResearch Laboratories | Cat #711-545-152 |
| Alexa Fluor® 647 Affinipure Donkey anti-mouse IgG | Jackson ImmunoResearch Laboratories | Cat #715-605-151 |
| Bacterial and virus strains | ||
| Sendai Virus Cantell Strain | ATCC | Cat #VR-907 |
| Sendai Virus Cantell Strain | Charles River Laboratories | Cat #VR-907 |
| Encephalomyocarditis virus (Cell culture adapted) | ATCC | Cat #VR-1762™ |
| IAVΔNS1 | Laboratory of Benjamin TenOever | Benjamin TenOever lab (Blanco-Melo et al., 2020) |
| VSV M51R | Laboratory of Benjamin TenOever | Benjamin TenOever lab |
| Chemicals, peptides, and recombinant proteins | ||
| Lonza NucleofectorTM 2b | Lonza Bioscience | Cat #: AAB-1001 |
| Nucleofector Kit L | Lonza Bioscience | Cat #VCA-1005 |
| Mammalian Protease Arrest | G-Biosciences | Cat #768-108 |
| TnT T7 Coupled Reticulocyte Lysate System | Promega | Cat #L4610 |
| Thapsigargin | Selleck Chem | Cat #S7895 |
| Puromycin | Selleck Chem | Cat #S7417 |
| CellEventTM Caspase 3-7 Green detection reagent | Invitrogen | Cat #C10423 |
| Hoechst 33342 | Thermo Fisher | Cat #H3570 |
| DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) | Thermo Fisher | Cat #D1306 |
| Fluoromount G | SouthernBiotech | Cat#0100-01 |
| Etoposide | Selleck Chem | Cat #S1225 |
| Staurosporine | Selleck Chem | Cat #S1421 |
| Z-IETD-FMK | Selleck Chem | Cat #S7314 |
| Human TNF-α | Cell Signaling | Cat #8902 |
| Q-VD-Oph | Selleck Chem | Cat #S7311 |
| Human TNF-α Neutralizing Rabbit mAb | Cell Signaling | Cat #7321 |
| Lipofectamine™ RNAiMAX Transfection Reagent | Invitrogen | Cat #13778150 |
| Recombinant IFN-β | Peprotech | Cat #300-02BC |
| Doxycycline | Fisher Scientific | Cat #AC446060050 |
| Cycloheximide | Selleck Chem | Cat#S7418 |
| Calf intestinal phosphatase (CIP) | New England Biolabs | Cat #M0525S |
| 2’-3’-cGAMP | Invivogen | Cat #tlrl-nacga23 |
| Tetraethylthiuram disulfide (Disulfiram) | Sigma-Aldrich | Cat #86720 |
| Necrosulfonamide | Sigma-Aldrich | Cat #480073 |
| Ruxolitinib | Selleck Chem | Cat #S1378 |
| BX-795 | Selleck Chem | Cat #S1274 |
| ACHP | Fisher Scientific | Cat#45-471-0 |
| Bengamide B | Fisher Scientific | Cat#52-731-00U |
| Pierce™ Sodium meta-periodate | Thermo Fisher | Cat #20504 |
| Zeba desalting columns | Thermo Fisher | Cat #89882 |
| Alt-R® S.p. Cas9 Nuclease | IDT | Cat #1081058 |
| Neon™ Transfection System | Invitrogen | Cat # MPK5000 |
| Neon™ Transfection System 10 μL Kit | Invitrogen | Cat #MPK1025 |
| Lipofectamine 3000 | Invitrogen | Cat #L3000001 |
| Digitonin | Calbiochem | Cat #CAS 11024-24-1 |
| Amersham ECL Prime Western Blotting Detection Reagent | Cytiva Life Sciences | Cat#RPN2236 |
| SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Fisher | Cat #34094 |
| 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels, 15-well | Bio-Rad | Cat #4561086 |
| TRI reagent (Trizol) | Zymo Research | Cat #R2050-1-200 |
| RNA Clean & Concentrator | Zymo Research | Cat #R1017 |
| High-Capacity cDNA reverse transcription kit | Applied Biosystems | Cat #4368813 |
| RNase Inhibitor, Human Placenta | New England Biolabs | Cat#M0307L |
| SYBR Green Master Mix | Applied Biosystems | Cat #4309155 |
| DMEM, high glucose, pyruvate | Cellgro | Cat #10-013-CV |
| DMEM, no glucose, no glutamine, no phenol red | Gibco | Cat #A1443001 |
| Airway epithelial cell basal medium | ATCC | Cat #PCS-300-030 |
| Bronchial epithelial cell growth kit | ATCC | Cat #PCS-300-040™ |
| Direct-zol RNA Miniprep kit | Zymo Research | Cat #R2052 |
| OPTI-MEM | Gibco | Cat #31985070 |
| Critical commercial assays | ||
| LumiKine™ Xpress hIFN-β 2.0 | Invivogen | Cat code luex-hifnbv2 |
| Human CCL5/RANTES Quantikine ELISA Kit | R&D systems | Cat #DRN00B |
| Human IL-6 Quantikine ELISA Kit | R&D systems | Cat #D6050 |
| Human TNF-alpha Quantikine ELISA Kit | R&D systems | Cat #DTA00D |
| Live/Dead ™ cell imaging kit | Thermo Fisher | Cat #R37601 |
| Sytox Green Nucleic Acid stain | Thermo Fisher | Cat #S7020 |
| Deposited Data | ||
| Raw data from RNA-seq | This study | GSE173953 |
| Raw imaging data | This study | 10.17632/ndwddpcyzm.1 |
| Experimental models: Cell lines | ||
| U2OS (WT, ΔG3BPs, ΔPKR, ΔUBAP2L) | Laboratory of Dr. Paul J. Anderson | Paul Anderson lab (Aulas et al., 2017; Kedersha et al., 2016; Sanders et al., 2020) |
| U2OS (ΔMAVS, ΔRIG-I, ΔG3BPs ΔRNase L, ΔPKR, ΔRNase L, ΔG3BPs ΔIRF3 and ΔG3BPs ΔPKR) | This study | N/A |
| A549 WT | Laboratory of Dr. Susan Weiss | Susan Weiss lab (Li et al., 2017) |
| A549 (ΔG3BPs, ΔG3BP ΔMAVS and ΔG3BPs ΔRNase L) | This study | N/A |
| HBEC3-KT | ATCC | CRL-4051 |
| HBEC3ΔG3BPs | This study | N/A |
| HeLa WT | Laboratory of Dr. Gracjan Michlewski | Gracjan Michlewski lab (Choudhury et al., 2014) |
| HeLaΔG3BPs | This study | N/A |
| HEK293T | ATCC | CRL-11268 |
| Oligonucleotides | ||
| 112 or 162 bp dsRNA | In-house | (Cadena et al., 2019) |
| Cy5-Monohydrazide | Cytiva | Cat #PA15121 |
| Poly I:C | Invivogen | Cat #tlrl-pic |
| ON-TARGETplus Human ADAR1 (103) siRNA Set | Horizon Discovery | Cat #LQ-008630-00-0020 |
| ON-TARGETplus Non-targeting Pool | Horizon Discovery | Cat #001810-10-20 |
| Primer sequences | See table S1 | N/A |
| Recombinant DNA | ||
| plentiCRISPRv2 | Addgene | Cat #98290 |
| psPAX2 | In-house | James DeCaprio lab (Cheng et al., 2017) |
| pMD2.G VSV-G | In-house | James DeCaprio lab (Cheng et al., 2017) |
| pInducer20 | In-house | Hidde Ploegh Lab (Ashour et al., 2015) |
| pInducer20-hG3BP1-IRES-hG3BP2 | This study | N/A |
| pFLAG-CMV4 empty vector | In-house | Ahmad et al., 2018 |
| pFLAG-CMV4-MDA5 (R337G) | In-house | Ahmad et al., 2018 |
| pFLAG-CMV4-MDA5 (G495R) | In-house | Ahmad et al., 2018 |