FIG. 1.

Anti-15-kDa antigen-specific MAbs and the HPA lectin decorate gliding sporozoites and identify shed antigen trails. Sporozoites locomoting on poly-l-lysine-coated slides were formaldehyde fixed, blocked, and incubated with MAb 11A5 (A), CrA1 (B), or CrA2 (C) or the biotin-conjugated lectin HPA (D). Reactivity profiles were visualized and documented by epifluorescence photomicroscopy following incubation of the washed slides with biotinylated secondary antibodies and/or Cy3-conjugated streptavidin.