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. 2000 Jul;68(7):4117–4134. doi: 10.1128/iai.68.7.4117-4134.2000

FIG. 2.

FIG. 2

MAbs 11A5 and CrA2 recognize the same 15-kDa glycoprotein antigen. Triton X-100-soluble oocyst/sporozoite proteins were either directly fractionated and blotted following SDS-PAGE (A and B, lanes 1) or fractionated and blotted following immunoprecipitation with IgG MAb 11A5 (A and B, lanes 2 and 4) or IgA MAb CrA2 (A and B, lanes 3 and 5). The blots were probed with primary MAb 11A5 (A, lanes 1 to 3) or primary MAb CrA2 (B, lanes 1 to 3) followed by alkaline phosphatase-conjugated secondary anti-IgG (A) or anti-IgA (B) antibodies. The arrow indicates the position of the 15-kDa antigen, the arrowhead points to the 25-kDa antigen, the asterisk denotes the 50-kDa antigen, and lanes 4 and 5 in both panels are secondary antibody controls (lacking primary antibody) which reveal the position of the precipitating MAb 11A5 and CrA2 heavy and light chains.