FIG. 3.

REAs affinity purified on plaque lifts of gDNA clones G4C1A and G7A1A recognize a 15-kDa C. parvum antigen. Oocyst lysates were fractionated by SDS-PAGE, blotted, and probed with REA prepared using gDNA (G) and cDNA (S) clones G9A1A (a), G7A1A (b), S10D1A (c), G4D1A (d), G15A1A (e), S9C1A (f), G12B1A (g), G4C1A (h), G1A1A (i), S5F1A (j), and S5C1A (k). The blots in lanes l and m were probed with REAs prepared on plaque lifts of the wild-type λZAP II and λgt11 phage vectors, respectively. Positive control blots were probed with MAbs 11A5 (n), CrA1 (o), and CrA2 (p); the position of the 15-kDa antigen is indicated by the arrowhead.