FIG. 4.

Nucleotide and deduced amino acid sequence of the NINC1 C. parvum gp15 gene. Locations of the gp15ATG and gp15STOP primers are indicated by arrows above and below the nucleotide sequence. The empirically determined N-terminal amino acid sequences of the HPA-affinity purified 15- and 45-kDa proteins are shown enclosed in boxes. The predicted N-terminal signal peptide cleavage site is indicated by the triangle, and the predicted GPI anchor attachment site is circled. Amino acid residues predicted to be O-glycosylated are subscripted by circles, and the single predicted N-glycosylated asparagine residue (Asn202) is boxed.