Fig. 3. Ube2g2-deficiency impairs biogenesis of virus replication organelles.

a Wild-type and Ube2g2^−/− cells were infected with ZIKV at MOI = 2. The expression levels of structural and non-structural proteins were analysed by Western blotting. Data is representative of four biologically independent samples. b Schematic of pulse chase assay. Cells were harvested following infection for 12, 24 or 48 h. Cells were starved of cysteine/methionine for 30 min and pulsed with 20µCi of [³⁵S]cysteine/methionine for 10 min, followed by chase in cold media for indicated timepoints. Supernatants and cell pellets were collected at each time point for immunoprecipitation. c Released virus particles were collected from supernatants (from 48 h post infection) by immunoprecipitation on anti-E antibodies, subjected to non-reducing versus reducing (+βME) conditions, resolved by gel electrophoresis and detected by autoradiography. d–f Lysates from indicated cells were subjected to immunoprecipitation on anti-E, anti-NS4B and anti-NS2B for autoradiography. g Quantitation of indicated viral proteins from pulse-chase assays. Values are representative of at least four independent biological experiments. h Viral prM immunoprecipitated from radiolabelled cells under non-reducing conditions and detected by autoradiography. All images are representative of at least 3 independent biological replicates. (Source data are provided as a Source data file).