Fig. 4. Ube2g2 is required for virus secretion.

a Schematic of virus like particles. b HeLa cells constitutively secreting virus like particles (VLPs) were subjected to control and Ube2g2 targeting siRNA. Lysates and supernatants from cells were immunoblotted for viral prM and E proteins. c Quantitation of viral prM and E proteins in the lysates and supernatants of wild-type and Ube2g2-depleted cells. Error bars represent mean ± SD, n = 3 biologically independent samples; **P < 0.01; ***P < 0.001 (compared with wild-type by ANOVA followed by one-sided Dunnett’s test). d VLP-secreting cells were treated with non-targeting and Ube2g2 targeting siRNA followed by Nile red staining for lipid droplets and anti-E staining for VLPs. e Abundance of lipid droplets was quantified by Nile red positive area over 500 cells. Error bars represent mean ± SD of relative LD abundance as % of control cells, n = 4 biologically independent samples. **P < 0.01; ***P < 0.001 (compared with wild-type by ANOVA followed by one-sided Dunnett’s test). f Wild-type and Ube2g2^−/− cells expressing vesicular stomatitis virus glycoprotein were pulsed with [³⁵S]cysteine/methionine and chased in cold media for indicated time intervals. VSV-G was immunoprecipitated at each time point and treated with EndoH at 37 °C for 1 h, resolved by SDS-PAGE and detected by autoradiography. Image is representative of 3 independent experiments. g Quantitation of EndoH-resistant forms of VSVG was determined from densitometric analyses of autoradiograms. Data is presented as mean ± SD, n = 3 biologically independent samples. (Source data are provided as a Source data file).