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. 2023 May 10;14:2698. doi: 10.1038/s41467-023-38377-w

Table 1.

Oligonucleotides used in this study

Primers ZIKV envelope primers

Fwd: 5’-TTGGGTTGTGTACGGAACCTG-3’

Rev: 5’-GTGCTTTGTGTATTCTCTTGA-3’

GAPDH primers

Fwd: 5’-GGAGCGAGATCCCTCCAAAAT-3’

Rev: 5’-GGCTGTTGTCATACTTCTCATGG-3’

primer (cloning)

Fwd (no-FLAG): 5’-ATATGGATCCGCCACC

ATGGCGGGGACCGCGCTCAAG-3’

Rev: 5’-CACGCTCGAGTCACAGTCCCAGAGACTTCT

GGACGATC-3’

Spliced Xbp1 (set 1)

Fwd: 5’-TGC TGA GTC CGC AGC AGG TG-3’

Rev: 5’-GCT GGC AGG CTC TGG GGA AG-3’

Spliced Xbp1 (set 2)

Fwd: 5’-GCT GAG TCC GCA GCA GGT-3’

Rev: 5’-CTG GGT CCA AGT TGT CCA GAA T-3’

Total Xbp1

Fwd: 5’- TGA AAA ACA GAG TAG CAG CTC AGA −3’

Rev: 5’-CTG GGT CCA AGT TGT CCA GAA T-3’

sgRNA ube2g2_A1_s CACCG-GTTGGGATGAAACATCTCAC
ube2g2_A1_a AAACGTGAGATGTTTCATCCCAACC