Table 1.
Primers | ZIKV envelope primers |
Fwd: 5’-TTGGGTTGTGTACGGAACCTG-3’ Rev: 5’-GTGCTTTGTGTATTCTCTTGA-3’ |
GAPDH primers |
Fwd: 5’-GGAGCGAGATCCCTCCAAAAT-3’ Rev: 5’-GGCTGTTGTCATACTTCTCATGG-3’ |
|
primer (cloning) |
Fwd (no-FLAG): 5’-ATATGGATCCGCCACC ATGGCGGGGACCGCGCTCAAG-3’ Rev: 5’-CACGCTCGAGTCACAGTCCCAGAGACTTCT GGACGATC-3’ |
|
Spliced Xbp1 (set 1) |
Fwd: 5’-TGC TGA GTC CGC AGC AGG TG-3’ Rev: 5’-GCT GGC AGG CTC TGG GGA AG-3’ |
|
Spliced Xbp1 (set 2) |
Fwd: 5’-GCT GAG TCC GCA GCA GGT-3’ Rev: 5’-CTG GGT CCA AGT TGT CCA GAA T-3’ |
|
Total Xbp1 |
Fwd: 5’- TGA AAA ACA GAG TAG CAG CTC AGA −3’ Rev: 5’-CTG GGT CCA AGT TGT CCA GAA T-3’ |
|
sgRNA | ube2g2_A1_s | CACCG-GTTGGGATGAAACATCTCAC |
ube2g2_A1_a | AAACGTGAGATGTTTCATCCCAACC |