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. 2023 Apr 26;14:1181180. doi: 10.3389/fpls.2023.1181180

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Copyright © 2023 Kondo, Aoki, Hirai, Sagami, Ito, Tsuzuki and Sato

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of lipid X in Synechocystis. (A) TLC profiles of total lipids in Synechocystis (6803) and Synechococcus (7942) with a solvent system of hexane/diethyl ether/acetate (70:30:1 by vol.) (left panel), and that of lipid X prepared from Synechocystis by TLC, as in the left panel, with another solvent system of 100% toluene (right panel). Arrowheads indicate the position of lipid X. O, origin. TG indicates a marker TG. (B) Fatty acid compositions of total lipids (TL) and lipid X (X). MS spectrum of TLC-prepared lipid X (C) or that of a lipid fraction (retention time of 15-17 min, m/z 1000-1160), including lipid X, in total lipids (D) on LC-MS analysis in Synechocystis. (E) Quantitative behavior of lipid X based on constituent fatty acid contents of TLC-prepared lipid X in total lipids on GC-analysis. (F) Quantitative behavior of a lipid fraction (retention time of 15-17 min, m/z 1000-1160), including lipid X, on LC-MS analysis of total lipids relative to total lipids (retention time of 2-18 min, m/z 200-1200) during cell growth in an aeration culture, based on the signal intensities on LC-MS analysis. (G) TLC profiles of lipid X in total lipids from aeration-cultured cells subjected to nitrogen-, sulfur- or phosphorus-deficiency (-N, -S or -P) stress, or 0.6 M NaCl stress. (H) Quantitative behavior of a lipid X fraction relative to total lipids in a static culture (SC) or in an aeration culture (AC), based on the signal intensities on LC-MS analysis, as in (F). The values shown are averages ± SD for three experiments. The significance of differences was evaluated by means of Student’s t-test. **P<0.05.