Figure 5.

Candidate alcohol that Slr2103 acts in lipid X synthesis. (A) MS chromatogram of m/z 748 on LC-MS analysis of total lipids in WT (blue) or Δslr2103 (red). The signal intensity of m/z 748 relative to the total lipid fraction in Δslr2103 was adjusted to 100%. Inset, the estimated relative signal intensity of m/z 748. (B) MS² spectrum of m/z 748 in Δslr2103. (C) MS chromatogram of m/z 748 on LC-MS analysis of total lipids in EV (blue) or OE (red) in Synechococcus. The signal intensity of m/z 748 relative to the total lipid fraction in EV was adjusted to 100%. Inset, the estimated relative signal intensities of m/z 748. (D) MS² spectrum of m/z 748 in EV. (E) High-resolution MS² spectrum of H⁺-adducted PQ-C in C reinhardtii. The structure of PQ-C with the hydroxy group at the first isoprene unit is tentatively shown. (F) High-resolution MS² spectrum of H⁺-adducted Y₇₆₅ in Synechocystis. The values shown are averages ± SD for three experiments. The significance of differences was evaluated by means of Student’s t-test. **P<0.05.