Figure 8.

Contribution of slr2103 to cell growth in Synechocystis in a static culture and its enhancement under saline stress conditions. (A) Effects of NaCl-stress on cell growth in the WT (open symbols) and Δslr2103 (closed symbols) in bubbling aeration cultures. Circles, 0 M NaCl. Triangles, 0.3 M NaCl. Squares, 0.6 M NaCl. (B) Cell growth of Δslr2103 in a static culture. Photographs of microtiter plates where the WT and Δslr2103 cells were statically cultured with or without NaCl stress at 0.3 to 0.9 M (C), and concomitant monitoring of cell growth based on the Chl content (D). Open and closed bars correspond to the non-floating (NF) and floating (F) cells, respectively. (E) Photographs of test tubes where the WT and Δslr2103 cells were statically cultured for 9 days with 0-0.6 M NaCl stress. (F) Quantitative behavior of lipid X relative to total lipids in statically cultured Synechocystis cells for 15 days with or without NaCl stress at 0.6 M, which was estimated as in Figure 1F . Indicated are the values for total (T), F or NF cells. Red dotted line, initial lipid X content in aeration-cultured cells before the shift to static conditions. The values shown are averages ± SD for three experiments. The significance of differences was evaluated by Student’s t-test. *P < 0.1. **P<0.05.