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Published in final edited form as: Circ Res. 2023 Mar 15;132(9):1127–1140. doi: 10.1161/CIRCRESAHA.122.321693

Figure 6. — Panel A. Effects of cross-linking (CL) of azido-biotinylated-cGMP (azido-BIO-cGMP) in isolated renal proximal tubules (RPT) from normal rats on αNKA protein recovery in streptavidin adsorbates. The experiment was repeated 3 separate times with new fresh kidneys for each condition measured (N=3). (1) Control: Opti-MEM. (2) Azido-BIO-cGMP CL: 2 μM. (3) Azido-BIO-cGMP Non-CL: 2 μM. Panel B. Effects of CL of azido-BIO-cGMP and azido-biotinylated-cAMP (azido-BIO-cAMP) on αNKA protein recovery. The experiment was repeated 8 separate times using new fresh kidneys every time (N=8). () Azido-BIO-cGMP CL: 2 μM. () Azido-BIO-cGMP Non-CL: 2 μM. () Azido-BIO-cAMP CL: 2 μM. () azido-BIO-cAMP Non-CL: 2 μM. Results are reported as percentages of azido-BIO-cGMP CL. Panel C. Effects of oubain (OUA) on CL of azido-BIO-cGMP in isolated RPTs on αNKA protein recovery. The experiment was repeated 6 separate times using new fresh kidneys every time for each condition measured (N=6). () Azido-BIO-cGMP CL: 2 μM. () Azido-BIO-cGMP Non-CL: 2 μM. () azido-BIO-cAMP CL + OUA: 10 μM. () azido-BIO-cGMP + OUA Non-CL: 10 μM. Results are reported as percentages of Azido-BIO-cGMP CL. Data represent mean ± 1 SE. Statistical significance was determined by using the repeated measures analysis with an unstructured covariance matrix in SAS PROC MIXED program. The ANOVA with permutation P value was based on 2,000 permutations of group assignment to individual N values and a repeated measures analysis with an unstructured covariance matrix.

Inline graphic — Panel A. Effects of cross-linking (CL) of azido-biotinylated-cGMP (azido-BIO-cGMP) in isolated renal proximal tubules (RPT) from normal rats on αNKA protein recovery in streptavidin adsorbates. The experiment was repeated 3 separate times with new fresh kidneys for each condition measured (N=3). (1) Control: Opti-MEM. (2) Azido-BIO-cGMP CL: 2 μM. (3) Azido-BIO-cGMP Non-CL: 2 μM. Panel B. Effects of CL of azido-BIO-cGMP and azido-biotinylated-cAMP (azido-BIO-cAMP) on αNKA protein recovery. The experiment was repeated 8 separate times using new fresh kidneys every time (N=8). () Azido-BIO-cGMP CL: 2 μM. () Azido-BIO-cGMP Non-CL: 2 μM. () Azido-BIO-cAMP CL: 2 μM. () azido-BIO-cAMP Non-CL: 2 μM. Results are reported as percentages of azido-BIO-cGMP CL. Panel C. Effects of oubain (OUA) on CL of azido-BIO-cGMP in isolated RPTs on αNKA protein recovery. The experiment was repeated 6 separate times using new fresh kidneys every time for each condition measured (N=6). () Azido-BIO-cGMP CL: 2 μM. () Azido-BIO-cGMP Non-CL: 2 μM. () azido-BIO-cAMP CL + OUA: 10 μM. () azido-BIO-cGMP + OUA Non-CL: 10 μM. Results are reported as percentages of Azido-BIO-cGMP CL. Data represent mean ± 1 SE. Statistical significance was determined by using the repeated measures analysis with an unstructured covariance matrix in SAS PROC MIXED program. The ANOVA with permutation P value was based on 2,000 permutations of group assignment to individual N values and a repeated measures analysis with an unstructured covariance matrix.