TABLE 4.

Liquid phase elution gradient

Time	A%	B%	Flow rate (ml/min)
0	5	95	0.5
0.5	5	95	0.5
7	35	65	0.5
8	60	40	0.5
9	60	40	0.5
9.1	5	95	0.5
12	5	95	0.5