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. 2023 May 10;19(5):e1010706. doi: 10.1371/journal.pgen.1010706

Fig 2. Effect of loss-of-function mutations in ELF3 and PHYC on the transcriptional profiles of six flowering time genes in 16h LD.

Fig 2

Normalized expression of (A) FT1, (B) PPD1, (C) CO1, (D) VRN1, (E) VRN2, and (F) CO2 during a 24h diurnal cycle in Bd21-3 (black line), elf3 (blue line), phyC (gray line) and elf3/phyC double mutant (orange line). Plants were grown in LDs until the fourth-leaf stage was reached (Zadoks = 14) at which point the newly expanded fourth leaf was harvested at zt0, zt4, zt8, zt12, zt16, and zt20. Note the zt0 value and zt24 value are the same. The average of four biological replicates is shown (three leaves per replicate). Error bars represent standard deviation of the mean. Data were normalized using UBC18 as done in [49].