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. 2023 May 10;19(5):e1010655. doi: 10.1371/journal.pgen.1010655

Fig 2. Transcript levels of PPD1 and FT1 in phyB and phyB elf3 mutants.

Fig 2

Samples were collected from leaves of 5-week-old Kronos photoperiod insensitive (PI) and sensitive (PS) plants grown under LD. (A-C) Wildtype vs. phyB in PI. (D-E) Wildtype vs. phyB in PS. (G-I) Wildtype vs. elf3 phyB in PI. (J-L) Wildtype vs. elf3 phyB in PS. (A & G) PPD-A1a allele with a deletion in the promoter. (D & J) PPD-A1b allele with intact promoter. (B, E, H, & K) PPD-B1 photoperiod sensitive allele. (C, F, I, & L) FT1 (both homoeologs). Expression in single elf3 mutants is discussed later in the ELF3 x PPD1 section. ACTIN was used as endogenous control. Error bars are s.e.m based on 5 biological replications. ns = not significant, * = P < 0.05, ** = P < 0.01, *** = P < 0.001 based on t-tests between mutants and wildtype at the different time points. Raw data and statistics are available in Data B in S1 Data.