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. 2023 May 10;9(19):eade7500. doi: 10.1126/sciadv.ade7500

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Fig. 4. — (A) Effect of U0126 on S43-METTL3 phosphorylation induced by EGF in HeLa cells. Western blot analysis of total cell extracts using the indicated antibodies. Blots in (A) to (D) are representatives of three biological repeats. (B) Pull-down of endogenous HEXIM1 from HeLa cell lysates using beads containing GST-tagged unphosphorylated or pS43 METTL3, produced in bacteria. Beads alone were used as a negative control. Western blot analysis using the indicated antibodies. Blots are a representation of three biological repeats. (C) IP of stably expressed Flag-METTL3. The top two panels represent the IP and the bottom three the input. Western blot analysis using the indicated antibodies. Wild-type HeLa cells were used as a negative control (NC) for the IP. (D) IP of stably expressed Flag-HEXIM1.