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. 2023 May 10;9(19):eadg1012. doi: 10.1126/sciadv.adg1012

Fig. 3. TaPGK was verified to regulate cold stress through mutants and overexpressed plants.

Fig. 3.

(A to D) Mutation sites of TaPGK (A) in Kronos EMS mutants and a comparison of phenotype, PGK activity, and pyruvate content of TaPGK EMS mutants and the WT (n = 6 plants per replicate) under cold stress (B to D). (E to H) Relative expression of TaPGK in TaPGK-overexpressed lines (E) and a comparison of phenotype, PGK activity, and pyruvate content of TaPGK-overexpressed lines and the WT (n = 6) under cold stress (F to H). The β-actin gene was served as the endogenous control. (I to L) Mutation sites of TaPGK (I) in gene edited (GE) Fielder mutants using CRISPER-Cas9 and comparison of phenotype, PGK activity, and pyruvate content of TaPGK-GE mutants and the WT (n = 6) under cold stress (J to L). gRNA, guide RNA. Experiments were conducted with three biological replicates. Values are presented as mean ± SE. **P < 0.01, as determined by paired Student’s t tests. KO, gene knockout. KO is the same to GE.